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使用荧光素-异硫氰酸酯标记的葡聚糖评估小鼠肠道屏障完整性
JoVE Journal
Biologia
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JoVE Journal Biologia
Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran
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使用荧光素-异硫氰酸酯标记的葡聚糖评估小鼠肠道屏障完整性

3,158 Views

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05:14 min

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November 18, 2022

DOI:

10.3791/64710-v

DOI:
10.3791/64710-v

•

05:14 min
November 18, 2022

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38 Views
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1Nutrition and Microbiome Laboratory, Institut du cancer de Montréal,Centre de recherche du Centre hospitalier de l’Université de Montréal (CRCHUM), 2Digestive Surgery Service, Centre hospitalier de l’Université de Montréal (CHUM), Faculty of Medicine,Université de Montréal, 3Department of Medicine, Faculty of Medicine,Université de Montréal

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FITC-dextranGut Barrier IntegrityIntestinal PermeabilityIn VivoNon-invasiveOral GavageFluorescence ImagingFecalPlasma

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Gerkins, C., Hajjar, R., Oliero, M., Santos, M. M. Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran. J. Vis. Exp. (189), e64710, doi:10.3791/64710 (2022).

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