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流式细胞术从小鼠组织中鉴定和分离爆破形成单元和集落形成单元红系祖细胞
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry
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流式细胞术从小鼠组织中鉴定和分离爆破形成单元和集落形成单元红系祖细胞

DOI:
10.3791/64373-v

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08:31 min

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November 04, 2022

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, , ,
1Department of Molecular, Cell and Cancer Biology,UMASS Chan Medical School

Capítulos

  • 00:05Introduction
  • 00:47Bone Marrow Cell Extraction
  • 02:07Spleen Cell Extraction
  • 02:46Antibody Staining of FMOs and Single-Color Controls
  • 03:53Cell Resuspension
  • 04:38Sample Preparation for Flow Cytometric Analysis
  • 05:19Gating Strategy for Setting up the Sorting Gates
  • 06:49Results: Effect of Erythropoietin on Bone Marrow and Spleen Progenitors
  • 07:42Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们描述了一种新的流式细胞术方法，用于直接从新鲜小鼠骨髓和脾脏中前瞻性分离早期爆发形成单位红系（BFU-e）和集落形成单位红系（CFU-e）祖细胞。该协议基于单细胞转录组学数据开发，是第一个以高纯度分离所有组织红系祖细胞的方案。

Tags

Burst-Forming Unit (BFU-E)Colony-Forming Unit (CFU-E)Erythroid ProgenitorsFlow CytometryBone MarrowSpleenCell StainingFluorescence Minus One (FMO) ControlsSingle-Color Controls

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