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荧光RNA适配体 外和细胞 开启动力学的测定
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Bioquímica
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JoVE Journal Bioquímica
Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
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荧光RNA适配体 外和细胞 开启动力学的测定

1,600 Views

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08:11 min

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August 09, 2022

DOI:

10.3791/64367-v

DOI:
10.3791/64367-v

•

08:11 min
August 09, 2022

•

7 Views
, ,
1Department of Chemistry and Henry Eyring Center for Cell & Genome Science,University of Utah

Summary

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该协议提供了两种确定荧光RNA适配体菠菜2和西兰花动力学的方法。第一种方法描述了如何使用酶标仪 在体外 测量荧光适配体动力学，而第二种方法详细介绍了通过流式细胞术测量细胞中荧光适配体动力学。

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Cite this Article

Mumbleau, M. M., Meyer, M. R., Hammond, M. C. Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers. J. Vis. Exp. (186), e64367, doi:10.3791/64367 (2022).

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