Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Efficient Transfection of In vitro Transcribed…
JoVE Journal
Immunology and Infection

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Immunology and Infection
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
 

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Article DOI: 10.3791/64288-v • 10:16 min • August 17th, 2022
August 17th, 2022 •
*1, *1, 1, 1, 1, 1, 1, 2, 1, 3, 1
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, Third Military Medical University, 2Department of Pediatrics, Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital, Third Military Medical University
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

A self-assembled peptide-poloxamine nanoparticle (PP-sNp) is developed using a microfluidic mixing device to encapsulate and deliver in vitro transcribed messenger RNA. The described mRNA/PP-sNp could efficiently transfect cultured cells in vitro.

Tags

Peptide-poloxamine Nanoparticles MRNA Delivery System Efficient Transfection In Vitro Transcribed MRNA Cultured Cells Mucosal MRNA Vaccine Low-cost Materials Short Protocol Bronchial Epithelial Cells Mucosal COVID-19 Vaccines Lung Cancers Cystic Fibrosis Gene Therapy Metridia Luciferase PUC57 Vector Linearized DNA Template T7 Transcription Kit Pseudouridine Uncapped IVT MRNA
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter