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新生児大腸 分離株の腸管トランスサイトーシスの評価
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates
DOI:

08:32 min

February 17, 2023

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Capítulos

  • 00:04Introduction
  • 00:44Preparing Transcytosis Inserts with T84 Cells (Approximately 1 – 2 Weeks Before the Experiment)
  • 03:43Preparing T84 Cells 1 Day Before the Experiment Using TCM w/o Antibiotics and Escherichia coli Cultures (Started 1 Day Before the Experiment)
  • 04:19Preparing E. coli Inoculum, Epithelial Cells, and Materials (On the Morning of the Experiment)
  • 05:05Inoculation of the Cells (Start of the Experiment)
  • 05:54Quantifying the Transcytosis and End of the Experiment
  • 06:56Results: Transcytosis of Neonatal E. coli Isolates
  • 08:04Conclusion

Summary

Tadução automática

大腸菌は、出生前後に細菌を摂取した新生児に敗血症を引き起こします。大腸が腸管から血流に移動する能力に関与するプロセスは、よく理解されていません。このin vitroモデルは、大腸菌株が腸上皮細胞を通過する能力を評価します。

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