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マウスアストロサイトの単離と直接ニューロンリプログラミング
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes
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マウスアストロサイトの単離と直接ニューロンリプログラミング

2,395 Views

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07:25 min

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July 07, 2022

DOI:

10.3791/64175-v

DOI:
10.3791/64175-v

•

07:25 min
July 07, 2022

•

6 Views
, ,
1Institute of Stem Cell Research, Helmholtz Zentrum München,German Research Center for Environmental Health, 2Department of Physiological Genomics, Biomedical Center Munich,Ludwig-Maximilians University, 3Graduate School of Systemic Neurosciences, BioCenter,Ludwig-Maximilians University

Summary

Automatically generated

ここでは、出生後マウスの中枢神経系のさまざまな領域に由来するアストロサイトの高度に濃縮された培養物を生成し、転写因子の強制発現によって機能的ニューロンに直接変換するための詳細なプロトコルについて説明します。

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Cite this Article

Hersbach, B. A., Simon, T., Masserdotti, G. Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes. J. Vis. Exp. (185), e64175, doi:10.3791/64175 (2022).

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