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Isolement et reprogrammation neuronale directe des astrocytes de souris
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Neurociência
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JoVE Journal Neurociência
Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes
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Isolement et reprogrammation neuronale directe des astrocytes de souris

2,395 Views

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07:25 min

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July 07, 2022

DOI:

10.3791/64175-v

DOI:
10.3791/64175-v

•

07:25 min
July 07, 2022

•

6 Views
, ,
1Institute of Stem Cell Research, Helmholtz Zentrum München,German Research Center for Environmental Health, 2Department of Physiological Genomics, Biomedical Center Munich,Ludwig-Maximilians University, 3Graduate School of Systemic Neurosciences, BioCenter,Ludwig-Maximilians University

Summary

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Nous décrivons ici un protocole détaillé pour générer des cultures hautement enrichies d’astrocytes dérivés de différentes régions du système nerveux central de souris postnatales et leur conversion directe en neurones fonctionnels par l’expression forcée de facteurs de transcription.

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Cite this Article

Hersbach, B. A., Simon, T., Masserdotti, G. Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes. J. Vis. Exp. (185), e64175, doi:10.3791/64175 (2022).

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