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Isolering og direkte neuronal omprogrammering af muse-astrocytter
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Neurociência
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JoVE Journal Neurociência
Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

Isolering og direkte neuronal omprogrammering af muse-astrocytter

2,395 Views

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07:25 min

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July 07, 2022

DOI:

10.3791/64175-v

DOI:
10.3791/64175-v

•

07:25 min
July 07, 2022

•

6 Views
, ,
1Institute of Stem Cell Research, Helmholtz Zentrum München,German Research Center for Environmental Health, 2Department of Physiological Genomics, Biomedical Center Munich,Ludwig-Maximilians University, 3Graduate School of Systemic Neurosciences, BioCenter,Ludwig-Maximilians University

Summary

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Her beskriver vi en detaljeret protokol til at generere højt berigede kulturer af astrocytter afledt af forskellige regioner i centralnervesystemet hos postnatale mus og deres direkte omdannelse til funktionelle neuroner ved tvungen ekspression af transkriptionsfaktorer.

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Cite this Article

Hersbach, B. A., Simon, T., Masserdotti, G. Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes. J. Vis. Exp. (185), e64175, doi:10.3791/64175 (2022).

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