Journal
/
Neurociência
/
Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

2,395 Views

•

07:25 min

•

July 07, 2022

DOI:

10.3791/64175-v

DOI:
10.3791/64175-v

•

07:25 min
July 07, 2022

•

6 Views
, ,
1Institute of Stem Cell Research, Helmholtz Zentrum München,German Research Center for Environmental Health, 2Department of Physiological Genomics, Biomedical Center Munich,Ludwig-Maximilians University, 3Graduate School of Systemic Neurosciences, BioCenter,Ludwig-Maximilians University

Summary

Automatically generated

Here we describe a detailed protocol to generate highly enriched cultures of astrocytes derived from different regions of the central nervous system of postnatal mice and their direct conversion into functional neurons by the forced expression of transcription factors.

Explore More Videos

AstrocytesNeuronal ReprogrammingCell IsolationSpinal CordCell CultureAstrocyte CulturePoly-D-LysineEGFBFGF

Read Article

Cite this Article

Hersbach, B. A., Simon, T., Masserdotti, G. Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes. J. Vis. Exp. (185), e64175, doi:10.3791/64175 (2022).

Download .ris file

Copy

Share Video

.

Copy