Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Single-Particle Cryo-EM Data Collection with…
JoVE Journal
Biochemistry

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Biochemistry
Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
 

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

Article DOI: 10.3791/64136-v • 04:52 min • July 1st, 2022
July 1st, 2022 •
1, 1, 2, 2,3, 1,4,5
1Laboratory of Genetics, The Salk Institute for Biological Studies, 2Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 3Department of Chemistry and Biochemistry, University of California San Diego, 4Graduate School of Biological Sciences, Section of Molecular Biology, University of California San Diego, 5Department of Integrative Structural and Computational Biology, The Scripps Research Institute

Capítulos

Resumo
Automatic Translation

The present protocol describes a generalized and easy-to-implement scheme for tilted single-particle data collection in cryo-EM experiments. Such a procedure is especially useful for obtaining a high-quality EM map for samples suffering from preferential orientation bias due to adherence to the air-water interface.

Tags

Single-Particle Cryo-EM Data Collection Stage Tilt Leginon Parallel Illumination Coma Aberrations Grid Atlas Square Acquisition Foil Integrity Ideal Ice Thickness Eucentric Height Alpha Wobbler Z Height Adjustment Image Shift Focuser Node Fine Z Focus Option
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter