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A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
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Bioquímica
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JoVE Journal Bioquímica
A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

DOI:
10.3791/64054-v

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12:27 min

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June 08, 2022

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, , , , , ,
1Division of Nephrology and Kidney Research Institute, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital,Sichuan University, 2Department of Neurosurgery, West China Hospital,Sichuan University

Capítulos

  • 00:05Introduction
  • 01:28Purification of Sphingosine 1-Phosphate Receptors (S1PRs)-G Protein Complex
  • 02:16Electron Microscopy to Resolve the S1PRs Structure
  • 07:56S1PRs-Gi Mediated cAMP Inhibition Assay
  • 10:33Results: Purification and Analysis of S1PRs-Gi Complex
  • 11:48Conclusion

Summary

Tadução automática

Tadução automática

S1P exerts its diverse physiological effects through the S1P receptors (S1PRs) subfamily. Here, a pipeline is described to expound on the structures and function of S1PRs.

Tags

Keywords: Sphingosine 1-phosphate ReceptorsS1PRActivationDrug DevelopmentCryo-electron MicroscopyProtein PurificationSize Exclusion Chromatography2D ClassificationImage ProcessingAuto-picking

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