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Interlinked Macroporous 3D Scaffolds from Microgel Rods
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JoVE Journal Bioengenharia
Interlinked Macroporous 3D Scaffolds from Microgel Rods

Interlinked Macroporous 3D Scaffolds from Microgel Rods

DOI:
10.3791/64010-v

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07:32 min

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June 16, 2022

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, , ,
1DWI – Leibniz Institute for Interactive Materials, 2Institute for Technical and Macromolecular Chemistry,RWTH Aachen University, 3Institute of Applied Medical Engineering, Department of Advanced Materials for Biomedicine,RWTH Aachen University

Capítulos

  • 00:05Introduction
  • 01:11Production and Purification of Amine and Epoxy Functionalized Microgel Rods
  • 03:34Macroporous Scaffold Formation and Cell Adhesive Post-Modification
  • 04:24Sterilization and Transfer into Cell Culture Media
  • 05:02Results: Interlinked Macroporous 3D Scaffolds from Microgel Rods
  • 06:41Conclusion

Summary

Tadução automática

Tadução automática

Microgel rods with complementary reactive groups are produced via microfluidics with the ability to interlink in aqueous solution. The anisometric microgels jam and interlink into stable constructs with larger pores compared to spherical-based systems. Microgels modified with GRGDS-PC form macroporous 3D constructs that can be used for cell culture.

Tags

Keywords: 3D ScaffoldsMicrogel RodsMicroporousInterlinkedCell-cell InteractionHigh Aspect RatioBlood Vessel FormationMicrofluidic FabricationUV Crosslinking

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