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A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
JoVE Journal
Neurociência
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JoVE Journal Neurociência
A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

2,614 Views

07:20 min

May 10, 2022

DOI:

07:20 min
May 10, 2022

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Summary

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The protocol provides a detailed method of neuronal imaging in brain slice using a tissue clearing method, ScaleSF. The protocol includes brain tissue preparation, tissue clarification, handling of cleared slices and confocal laser scanning microscopy imaging of neuronal structures from mesoscopic to microscopic levels.

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