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从小鼠面部过程和培养的腭间充质细胞中分离全细胞蛋白质裂解物以进行磷酸化蛋白分析
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
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从小鼠面部过程和培养的腭间充质细胞中分离全细胞蛋白质裂解物以进行磷酸化蛋白分析

DOI:
10.3791/63834-v

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07:26 min

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April 01, 2022

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, ,
1Department of Craniofacial Biology, School of Dental Medicine,University of Colorado Anschutz Medical Campus

Capítulos

  • 00:04Introduction
  • 00:48Harvesting E11.5 Mouse Embryos
  • 02:31Dissecting Maxillary Processes from E11.5 Mouse Embryos
  • 04:12Isolating Whole Cell Protein Lysates from Mouse Maxillary Processes
  • 05:06Isolating Whole Cell Protein Lysates from Primary and/or Immortalized Mouse Embryonic Palatal Mesenchyme Cells
  • 06:22Results: Assessing Phosphorylated Protein Levels in Cell Lysates from Cultured Mouse Embryonic Palatal Mesenchyme Cells
  • 06:59Conclusion

Summary

Tadução automática

Tadução automática

该协议提出了一种从解剖的小鼠胚胎面部过程或培养的小鼠胚胎腭间充质细胞中分离全细胞蛋白裂解物并随后进行蛋白质免疫印迹以评估磷酸化蛋白水平的方法。

Tags

Protein LysatesPhosphoprotein AnalysisFacial ProcessesPalatal Mesenchyme CellsCraniofacial DevelopmentMouse EmbryosProtein IsolationPhosphatase InhibitorsProtein PhosphorylationDissectionCell Culture

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