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In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility
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Neurociência
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JoVE Journal Neurociência
In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility

In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility

DOI:
10.3791/63782-v

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07:35 min

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April 21, 2022

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, , , ,
1Department of Pathology & Cell Biology; Vagelos College of Physicians and Surgeons,Columbia University, 2Department of Molecular Pharmacology and Therapeutics; Vagelos College of Physicians and Surgeons,Columbia University, 3Department of Neurology; Vagelos College of Physicians and Surgeons,Columbia University, 4The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain; Vagelos College of Physicians and Surgeons,Columbia University

Capítulos

  • 00:04Introduction
  • 00:44OCT and Fluorescein Imaging
  • 02:08Fluorescein Image Processing
  • 03:26Retinal Layer Thickness
  • 04:01Disorganization of Retinal Inner Layers
  • 05:02Results: Quantification of FA and OCT Images in the Study of Retinal Vein Occlusion
  • 06:46Conclusion

Summary

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Here, we present three data analysis protocols for fluorescein angiography (FA) and optical coherence tomography (OCT) images in the study of Retinal Vein Occlusion (RVO).

Tags

Keywords: Retinal ImagingVascular InjuryMouse ModelOptical Coherence TomographyFluorescein LeakageRetinal Layer Analysis

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