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Purificazione e identificazione di proteine specifiche per tipo cellulare da tessuti complessi utilizzando una linea murina di topo di metionina tRNA sintetasi mutante
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Neurociência
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JoVE Journal Neurociência
Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line
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Purificazione e identificazione di proteine specifiche per tipo cellulare da tessuti complessi utilizzando una linea murina di topo di metionina tRNA sintetasi mutante

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07:39 min

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April 13, 2022

DOI:

10.3791/63713-v

DOI:
10.3791/63713-v

•

07:39 min
April 13, 2022

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4 Views
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1Max-Planck-Institute for Brain Research,Synaptic Plasticity Department, 2Department of Biochemistry and Molecular Biology,Veterinary School, Complutense University of Madrid

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Cell-type SpecificProtein PurificationProtein IdentificationMutant Methionine TRNA SynthetaseCell Type Specific ProteinsIn VitroIn VivoImmunofluorescentAlkylationIodoacetamideBuffer ExchangeClick ChemistryAzidonorleucine IncorporationNeutravidin Binding

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Nassim-Assir, B., Ouro Corredera, D., Alvarez-Pardo, R., tom Dieck, S., Ciirdaeva, E., Schuman, E. M., Alvarez-Castelao, B. Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line. J. Vis. Exp. (182), e63713, doi:10.3791/63713 (2022).

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