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La génétique inverse pour concevoir des variants du virus à ARN à sens positif
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JoVE Journal Genética
Reverse Genetics to Engineer Positive-Sense RNA Virus Variants
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La génétique inverse pour concevoir des variants du virus à ARN à sens positif

1,069 Views

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15:49 min

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June 09, 2022

DOI:

10.3791/63685-v

DOI:
10.3791/63685-v

•

15:49 min
June 09, 2022

•

2 Views
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1Virology Section, Department of Life Sciences,Shiv Nadar University, 2Gastroenterology,Sanjay Gandhi Postgraduate Institute of Medical Sciences

Tags

Reverse GeneticsPositive-sense RNA VirusViral GenomeMutagenesisRNA LibraryPhenotype SelectionCloning-freeEp-PCRIn Vitro RNA SynthesisCDNA SynthesisAmplificationA Overhang Addition

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Soni, S., Agarwal, S., Aggarwal, R., Veerapu, N. S. Reverse Genetics to Engineer Positive-Sense RNA Virus Variants. J. Vis. Exp. (184), e63685, doi:10.3791/63685 (2022).

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