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反向遗传学设计正义RNA病毒变异株

JoVE Journal
Genética
Author Produced

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JoVE Journal Genética

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

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反向遗传学设计正义RNA病毒变异株

1,069 Views

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15:49 min

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June 09, 2022

DOI:

10.3791/63685-v

DOI:

10.3791/63685-v

•

15:49 min

June 09, 2022

•

2 Views

Shalini Soni, Shubhra Agarwal, Rakesh Aggarwal, Naga Suresh Veerapu

¹Virology Section, Department of Life Sciences,Shiv Nadar University, ²Gastroenterology,Sanjay Gandhi Postgraduate Institute of Medical Sciences

Tags

Reverse Genetics Positive-sense RNA Virus Viral Genome Mutagenesis RNA Library Phenotype Selection Cloning-free Ep-PCR In Vitro RNA Synthesis CDNA Synthesis Amplification A Overhang Addition

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