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JoVE Journal Neuroscience
Expanding the Toolkit for In Vivo Imaging of Axonal Transport
 

Expanding the Toolkit for In Vivo Imaging of Axonal Transport

Article DOI: 10.3791/63471-v • 09:24 min • December 23rd, 2021
December 23rd, 2021 •
1,2, 1,2,3, 1,2,3, 1,2,3
1Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology, University College London, 2UCL Queen Square Motor Neuron Disease Centre, University College London, 3UK Dementia Research Institute, University College London

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Using transgenic fluorescent mice, detailed protocols are described to assess in vivo axonal transport of signaling endosomes and mitochondria within motor and sensory axons of the intact sciatic nerve in live animals.

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Keywords: In Vivo Imaging Axonal Transport Signaling Endosomes Mitochondria Peripheral Neurons Motor Disease Models Aging Glass Micropipette Intramuscular Injections Tibialis Anterior Muscle Tetanus Neurotoxin Sciatic Nerve
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