/

Biologia do Desenvolvimento

/

从小鼠垂体中开发类器官作为体外模型以探索垂体干细胞生物学

JoVE Journal
Biologia do Desenvolvimento

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia do Desenvolvimento

Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

Please note that all translations are automatically generated. Click here for the English version.

从小鼠垂体中开发类器官作为体外模型以探索垂体干细胞生物学

DOI:

10.3791/63431-v

•

09:48 min

•

February 25, 2022

•

Emma Laporte*¹, Charlotte Nys*¹, Hugo Vankelecom

¹Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, Leuven Stem Cell Institute,KU Leuven (University of Leuven)

Capítulos

00:04Introduction
00:53Isolation of Mouse Anterior Lobe
01:57Dissociation of Pituitary into Single Cells
04:28Establishment and Culturing of Anterior Lobe Derived Organoids
07:31Results: Establishment and Validation of Anterior Lobe Derived Organoids
09:21Conclusion

Summary

Tadução automática

垂体是人体内分泌系统的关键调节因子。本文描述了从小鼠垂体中开发类器官作为一种新的3D 体外模型，以研究腺体的干细胞群，其中的生物学和功能仍然知之甚少。

Tags

Keywords: Organoids Mouse Pituitary In Vitro Model Pituitary Stem Cell Biology Pituitary Remodeling Neonatal Maturation Aging Tumor Growth Cell Isolation Trypsin DNAse Cell Culture Medium

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

后生转换为安全和简单的方法来获得成人皮肤成纤维细胞胰岛素分泌细胞

新生小鼠胰腺胰岛功能的有效隔离

发育小鼠垂体的解剖和冠状切片制备

无支架三维胰岛素表达 Pancreatoids 的实验研究体外培养的小鼠胰祖细胞

全山荧光在小鼠胚胎发生过程中心脏室发育的分析

评估原小鼠眼部表面细胞/干细胞对紫外线-C （UV-C）损伤的反应

一种从人类胚胎干细胞中生成脑器官的静态自导方法

为早期小鼠胚胎测序准备小型RNA库

从人类诱导多能干细胞诱导的体外神经肌肉结点

从成年小鼠味干细胞中衍生的语言器官的生成与培养

Read Article