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Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

DOI:
10.3791/63348-v

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08:09 min

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August 29, 2022

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, , ,
1Biochemistry Department, Faculty of Pharmacy,Ain Shams University, 2Pharmacology and Biochemistry Department, Faculty of Pharmacy,The British University in Egypt, 3Center for Drug Research and Development (CDRD), Faculty of Pharmacy,The British University in Egypt

Capítulos

  • 00:12Introduction
  • 01:35Isolation of Ad-MSCs from Rat Epididymal Fat Pads
  • 06:37Resultados
  • 07:42Conclusions

Summary

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Adipose tissue-derived mesenchymal stem cells (Ad-MSCs) can be a potential source of MSCs that differentiate into insulin-producing cells (IPCs). In this protocol, we provide detailed steps for the isolation and characterization of rat epididymal Ad-MSCs, followed by a simple, short protocol for the generation of IPCs from the same rat Ad-MSCs.

Tags

Mesenchymal Stem CellsIsolationCharacterizationDifferentiationInsulin-producing CellsCollagenase DigestionCD MarkersMesodermal Lineages

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