É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
基于激光显微切割的协议，用于神经黑色素颗粒蛋白质组学谱的LC-MS / MS分析
1Medizinisches Proteom-Center, Medical Faculty,Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI),Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy,University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research,University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy,University Hospital of Wuerzburg, University of Wuerzburg
Capítulos
- 00:05Introduction
- 00:46Laser Microdissection and Pressure Catapulting
- 02:46Sample Preparation for HPLC-MS and Proteomic Raw Data Analysis
- 05:10Statistical Analysis
- 05:50Results: Protein Identification in Neuromelanin Granules (NMGs) and Substantia nigra (SN)
- 07:00Conclusion
