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High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
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Biologia
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JoVE Journal Biologia
High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

DOI:
10.3791/63202-v

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06:39 min

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April 21, 2022

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, , , ,
1National Center for Advancing Translational Sciences, 2Optical Sciences Division,Code 5600, Naval Research Laboratory, 3Jacobs Corporation

Capítulos

  • 00:04Introduction
  • 00:30Treatment of Cells with QD-Spike
  • 01:39Fixation and Nuclei Staining
  • 02:36Acquisition Set-Up and Imaging
  • 04:39Results: Imaging and Visualization of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers
  • 06:11Conclusion

Summary

Tadução automática

Tadução automática

In this protocol, quantum dots conjugated to recombinant SARS-CoV-2 spike enable cell-based assays to monitor spike binding to hACE2 at the plasma membrane and subsequent endocytosis of the bound proteins into the cytoplasm.

Tags

Keywords: Quantum DotSARS-CoV-2Spike TrimerBindingEndocytosisHEK293T CellsHigh-throughputConfocal ImagingVirus EntryCell RecognitionQuantum Dot ConjugationBSADilutionPFANuclear DyeImaging ProtocolMicroscopy

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