É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
尿嘧啶-DNA糖基化酶测定，采用基质辅助激光解吸/电离飞行时间质谱分析
3,629 Views
•
12:19 min
•
April 22, 2022
DOI:
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, 2Department of Laboratory Medicine,National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University
Summary
Automatically generated
使用MALDI-TOF质谱法开发了一种非标记，非放射性同位素方法来测定尿嘧啶-DNA糖基化酶活性，用于直接分析含阳离子/嘧啶位点的产品。该测定被证明是非常简单，特异性，快速且易于使用的DNA糖基化酶测量。
Read Article
Cite this Article
Chang, H., Su, K., Goodman, S. D., Cheng, W., Lin, L., Yang, Y., Chang, S., Fang, W. Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis. J. Vis. Exp. (182), e63089, doi:10.3791/63089 (2022).
Copy