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רשתות מיקרוסקופיות אלקטרוניות של שידור מיקרו-מפזרנינג למיקום תאים ישירים בתהליכי עבודה של טומוגרפיה קריו-אלקטרונים של תאים שלמים
This content is Free Access.
1Department of Biochemistry,University of Wisconsin, Madison, 2Cryo-Electron Microscopy Research Center, Department of Biochemistry,University of Wisconsin, Madison, 3Midwest Center for Cryo-Electron Tomography, Department of Biochemistry,University of Wisconsin, Madison, 4Department of Chemistry,University of Wisconsin, Madison, 5Morgridge Institute for Research
Capítulos
- 00:04Introduction
- 01:25Preparation of Grids for Patterning and Application of the Anti-fouling Layer
- 02:50Applying PLPP Gel
- 03:33Micropatterning
- 06:11Deposition of Extracellular Matrix (ECM) Proteins
- 06:54Results: Micropatterning to Direct Cell Positioning within Whole-cell Cryo-electron Tomography
- 09:17Conclusion
