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全卵巢免疫荧光、清除和多光子显微镜用于小鼠发育中卵巢储备的定量3D分析
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse
DOI:

12:36 min

September 03, 2021

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Capítulos

  • 00:04Introduction
  • 01:00Perfusion, Dissection, and Fixation of Pubertal Ovaries
  • 02:34Whole-Mount Ovary Immunostaining
  • 03:59Clearing of Immunostained Whole-Mount Ovaries
  • 04:54Sample Setup and Imaging with a Multiphoton Microscope
  • 06:54Oocyte Quantification
  • 09:28Results: Quantitative and Qualitative Analysis of the Ovaries Using Immunostaining, Multiphoton Microscopy, and 3D Visualization
  • 11:51Conclusion

Summary

Tadução automática

在这里,我们提出了一种优化的方案,用于对整个卵巢进行成像,以使用全装载免疫染色,多光子显微镜以及3D可视化和分析进行定量和定性分析。该方案适用于高通量、可靠和可重复的处理,适用于毒理学、临床诊断和卵巢功能的基因组测定。

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