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DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis
JoVE Journal
Medicina
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JoVE Journal Medicina
DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

2,963 Views

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12:39 min

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September 28, 2021

DOI:

10.3791/62941-v

DOI:
10.3791/62941-v

•

12:39 min
September 28, 2021

•

1 Views
, , , , , , ,
1Karolinska Institutet, 2Central European Institute of Technology

Summary

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Double resin casting micro-computed tomography, or DUCT, enables visualization, digitalization, and segmentation of two tubular systems simultaneously to facilitate 3D analysis of organ architecture. DUCT combines ex vivo injection of two radiopaque resins followed by micro-computed tomography scanning and segmentation of the tomographic data.

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Cite this Article

Hankeova, S., Salplachta, J., Van Hul, N., Kavkova, M., Iqbal, A., Zikmund, T., Kaiser, J., Andersson, E. R. DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis. J. Vis. Exp. (175), e62941, doi:10.3791/62941 (2021).

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