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使用遗传编码的Ca^{2 +} 指示剂（GECI）对星形胶质细胞和神经元中的线粒体Ca^{2 +} 摄取进行成像

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Neurociência
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JoVE Journal Neurociência

Imaging Mitochondrial Ca²⁺ Uptake in Astrocytes and Neurons using Genetically Encoded Ca²⁺ Indicators (GECIs)

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使用遗传编码的Ca^{2 +} 指示剂（GECI）对星形胶质细胞和神经元中的线粒体Ca^{2 +} 摄取进行成像

DOI:

10.3791/62917-v

•

07:46 min

•

January 22, 2022

•

Nannan Zhang, Zhe Zhang², Ilker Ozden, Shinghua Ding²

¹Dalton Cardiovascular Research Center,University of Missouri-Columbia, ²Department of Biomedical, Biological and Chemical Engineering,University of Missouri-Columbia

Capítulos

00:03Introduction
00:38Construction of DNA Plasmids
00:57In vitro Mitochondrial Ca²⁺ Imaging in Astrocytes and Neurons
01:32In vivo Mitochondrial Ca²⁺ Imaging in Astrocytes and Neurons
03:51Cranial Window Installation and in vivo 2-P Imaging of Mitochondrial Ca²⁺ Signals
05:42Representative Results of in vitro and in vivo Mitochondrial Ca²⁺ Imaging
07:24Conclusions

Summary

Tadução automática

该proctocol旨在为星形胶质细胞和神经元 中的体外 和体内线粒体Ca^{2 +} 成像提供一种方法。

Tags

Keywords: Mitochondrial Calcium Imaging Astrocytes Neurons Genetically Encoded Calcium Indicators (GECIs)GCaMP5G GCaMP6s In Vitro In Vivo Adenovirus Stereotaxic Injection Motor Cortex Paracortex

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