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Tijd-opgeloste Förster Resonantie Energie Transfer Assays voor meting van endogene gefosforyleerde STAT-eiwitten in menselijke cellen

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells

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Tijd-opgeloste Förster Resonantie Energie Transfer Assays voor meting van endogene gefosforyleerde STAT-eiwitten in menselijke cellen

3,202 Views

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07:12 min

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September 09, 2021

DOI:

10.3791/62915-v

DOI:

10.3791/62915-v

•

07:12 min

September 09, 2021

•

4 Views

Jaime Padros, Geneviève Chatel, Mireille Caron

¹BioAuxilium Research

Tags

Time-resolved FRET TR-FRET STAT Proteins JAK/STAT Signaling Immunoassay Cell Culture Cell Stimulation Cell Lysis Phosphatase Inhibitors High-throughput Screening Pharmacological Characterization

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