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Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

DOI:
10.3791/62818-v

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05:28 min

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August 19, 2021

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,
1Department of Molecular Cell Biology,University of California

Capítulos

  • 00:05Introduction
  • 00:45Global Light Induction
  • 01:26Quantitative Real-time PCR and Fluorescence Microscopy
  • 03:59Results: Light-activated Gene Expression Assessment in Zebrafish Embryo
  • 04:46Conclusion

Summary

Tadução automática

Tadução automática

Optogenetics is a powerful tool with wide-ranging applications. This protocol demonstrates how to achieve light-inducible gene expression in zebrafish embryos using the blue light-responsive TAEL/C120 system.

Tags

Light-induced GFP ExpressionOptogeneticTAEL/C120 SystemZebrafish EmbryosInducible Gene ExpressionLineage TracingGain-of-function AssaysRescue ExperimentsRNA ExtractionCDNA SynthesisQuantitative PCR

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