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Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
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Bioquímica
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JoVE Journal Bioquímica
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

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07:16 min

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June 21, 2021

DOI:

10.3791/62671-v

DOI:
10.3791/62671-v

•

07:16 min
June 21, 2021

•

2 Views
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1Integrative Omics, Biological Sciences Division,Pacific Northwest National Laboratory, 2Bioproducts Sciences and Engineering Laboratory, Department of Biological Systems Engineering,Washington State University

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Protein Thiol OxidationResin-assisted CaptureIsobaric Tandem Mass Tag LabelingQuantificationCystine ResiduesSample PreparationAcetone WashProtein SolubilizationBCA AssayCentrifugal FiltrationProtein ReductionDTTBuffer Exchange

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Gaffrey, M. J., Day, N. J., Li, X., Qian, W. Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation. J. Vis. Exp. (172), e62671, doi:10.3791/62671 (2021).

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