Journal
/
/
使用废培养基对人类植入前胚胎进行染色体筛选:样品采集和染色体倍性分析
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
DOI:

12:32 min

September 07, 2021

, , , , , , ,

Capítulos

  • 00:04Introduction
  • 01:24Preparation and Denudation of Granulosa Cells, Oocyte Evaluation and Embryo Washing
  • 03:15Culture Medium Collection Options 1 and 2, Sample Collection
  • 05:12Culture Medium Lysis
  • 06:22Pre‐Library Preparation
  • 07:24Library Preparation
  • 08:14Library Purification and Quantification, Sequencing and Data Analysis
  • 09:43Results: Embryo Screening and Selection Using NICS from a Patient
  • 11:08Conclusion

Summary

Tadução automática

本研究报告了一种使用废培养基对人类胚胎进行染色体筛选的方案,该方案避免了胚胎活检并使用 NGS 进行染色体倍性鉴定。本文介绍了详细的过程,包括培养基的制备、全基因组扩增(WGA)、下一代测序(NGS)文库的制备和数据分析。

Vídeos Relacionados

Read Article