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Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
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JoVE Journal Bioquímica
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

DOI:
10.3791/62589-v

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12:26 min

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February 12, 2022

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, ,
1Helmholtz Institute for RNA-based Infection Research (HIRI),Helmholtz Zentrum für Infektionsforschung (Helmholtz Centre for Infection Research), 2Medical Faculty,Julius-Maximilians University Würzburg

Capítulos

  • 00:02Introduction
  • 01:56Sample Preparation
  • 04:20Instrument Setup
  • 05:47Sample Measurement
  • 09:19Data Analysis
  • 10:06Representative Results
  • 12:18Conclusion

Summary

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This protocol presents a complete experimental workflow for studying RNA-protein interactions using optical tweezers. Several possible experimental setups are outlined including the combination of optical tweezers with confocal microscopy.

Tags

Keywords: Optical TweezersRNA-protein InteractionsTranslation RegulationSingle Molecule TechniquesFluorescence Assisted Optical TweezersForce-ramp ExperimentsConstant Force MeasurementsReal-time VisualizationDNA-RNA ConstructIn-vitro TranscriptionPCR Reactions

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