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从组织和尿液中产生人类肾脏结核

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Generation of Human Kidney Tubuloids from Tissue and Urine

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从组织和尿液中产生人类肾脏结核

DOI:

10.3791/62404-v

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08:34 min

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April 16, 2021

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Camilla Calandrini², Jarno Drost²

¹Princess Máxima Center for Pediatric Oncology, ²Oncode Institute

Capítulos

00:04Introduction
00:50Generation of Human Kidney Tubuloids From Tissue
03:45Generation of Human Kidney Tubuloids From Urine
05:02Expansion of Tubuloid Cultures
07:01Results: Successful Generation of Human Kidney Tubuloids From Tissue and Urine
07:55Conclusion

Summary

Tadução automática

人类肾结肠培养物是研究肾脏生理学和疾病的宝贵体外模型。甲状腺素可以从肾脏组织（健康和患病）以及尿液中建立，后者代表一种容易获得和侵入性较小的研究材料来源。

Tags

Keywords: Kidney Tubuloids Urine Kidney Disease Modeling Kidney Physiology Basement Membrane Extract Collagenase Cell Culture

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