Journal
/
Imunologia e Infecção
/
Analyse af HBV-specifikke CD4 T-celle svar og identifikation af HLA-DR-begrænset CD4 T-Cell Epitopes Baseret på en Peptid Matrix
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Analyse af HBV-specifikke CD4 T-celle svar og identifikation af HLA-DR-begrænset CD4 T-Cell Epitopes Baseret på en Peptid Matrix

DOI:
10.3791/62387-v

•

10:37 min

•

October 20, 2021

•

, , ,
1Department of Infectious Diseases,Southwest Hospital, Third Military Medical University (Army Medial University), 2Chongqing Key Laboratory for Research of Infectious Diseases

Capítulos

  • 00:04Introduction
  • 00:51Isolation of PBMCs and In Vitro Expansion Using an HBV Peptide Matrix
  • 03:55Analysis of HBV-specific CD4 T-Cell Responses by Intracellular Flow Cytometry
  • 05:54Identification of HBV-specific HLA-DR Restricted CD4 T-Cell Epitopes
  • 08:07Results: Identification of HBV-specific HLA-DR Restricted CD4 T-Cell Epitopes
  • 10:06Conclusion

Summary

Tadução automática

Tadução automática

Baseret på en hepatitis B-virus (HBV)-afledt peptidmatrix kan HBV-specifikke CD4 T-celleresponser evalueres parallelt med identifikation af HBV-specifikke CD4 T-celle epitoper.

Tags

Keywords: HBV-specific CD4 T-cell ResponsesHLA-DR-restricted CD4 T-cell EpitopesChronic HBV InfectionPBMC IsolationPeptide MatrixCD4 T-cell Response AnalysisHBV-derived Peptide PoolsIL-2IL-796-well Plates

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/62387?article_type=v

Retroviral Transduction of T-cell Receptors in Mouse T-cells
check_url/pt/62387?article_type=v

Anvendelse af interferon-γ Enzymbundet immunospot Assay til karakterisering hidtil ukendte T-celle epitoper af human papillomavirus
check_url/pt/62387?article_type=v

In vitro assay at evaluere virkningen af immunoregulerende Pathways på HIV-specifikke CD4 T-celle effektorfunktion
check_url/pt/62387?article_type=v

Anvendelse af fluorescerende Skud Arrays for Vurdering af T-celle respons<em> In vivo</em
check_url/pt/62387?article_type=v

Transplantation af Tail Hud til Study Allogen CD4 T-celle respons i mus
check_url/pt/62387?article_type=v

Mus Naïve CD4<sup> +</sup> T Cell Isolation og<em> In vitro</em> Differentiering til T-celledelmængder
check_url/pt/62387?article_type=v

Whole-dyr Imaging og Flowcytometrisk Teknikker til analyse af antigen-specifikke CD8 + T-celle responser efter Nanopartikel Vaccination
check_url/pt/62387?article_type=v

Imaging CD4 T-celle Interstitiel Migration i det betændte Dermis
check_url/pt/62387?article_type=v

En suge Blister protokol at studere Human T-celle hjemkalde svar In Vivo
check_url/pt/62387?article_type=v

Evaluering af Zika Virus-specifikke T-celle respons i Immunoprivileged organer af inficerede Ifnar1- / - mus

Read Article