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使用肠道器官模型研究猿特异性相互作用的文化方法
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models
DOI:

07:49 min

March 23, 2021

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Capítulos

  • 00:04Introduction
  • 00:32Inversion of Intestinal Organoid Polarity
  • 02:57Dissociating Intestinal Organoids for Monolayer Generation and Culture
  • 05:21Results I: Analysis and Characterisation of the Morphology and Cell Polarity of Intestinal Organoids
  • 06:27Results II: Analysis and Characterisation of the Morphology and Differentiation Markers of Intestinal Monolayers
  • 07:03Conclusion

Summary

Tadução automática

在这里,我们提出了两个协议,允许肠道特定的相互作用建模。器官衍生肠道单层和空气-液体接口(ALI)培养物有助于产生从亮面和玄武岩两侧获得分化良好的表皮,而极性倒置肠道器官暴露其腹腔侧,并适合高通量检测。

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