Journal
/
Imunologia e Infecção
/
Gene Knock-in durch CRISPR/Cas9 und Zellsortierung in Makrophagen- und T-Zelllinien
JoVE Journal
Imunologia e Infecção
Author Produced
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
Please note that all translations are automatically generated. Click here for the English version.

Gene Knock-in durch CRISPR/Cas9 und Zellsortierung in Makrophagen- und T-Zelllinien

9,722 Views

•

11:32 min

•

November 13, 2021

DOI:

10.3791/62328-v

DOI:
10.3791/62328-v

•

11:32 min
November 13, 2021

•

9 Views
, , , , , , , , ,
1The Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine,Xinxiang Medical University, 2Centre d’Immunologie de Marseille-Luminy,Aix Marseille Université

Tags

CRISPR/Cas9Gene Knock-inMacrophageT Cell LinesROSA26 LocusSgRNA DesignHomologous RecombinationTargeting VectorFluorescent ReporterCell Sorting

Read Article

Cite this Article

Zhang, L., Huang, R., Lu, L., Fu, R., Guo, G., Gu, Y., Liu, Z., He, L., Malissen, M., Liang, Y. Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines. J. Vis. Exp. (177), e62328, doi:10.3791/62328 (2021).

Download .ris file

Copy

Share Video

.

Copy