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High-Throughput Transcriptome Analyse til undersøgelse af Host-Patogen Interaktioner
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

High-Throughput Transcriptome Analyse til undersøgelse af Host-Patogen Interaktioner

DOI:
10.3791/62324-v

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14:58 min

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March 05, 2022

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, , , , ,
1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School,University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences,University of São Paulo, 4Hospital Israelita Albert Einstein

Capítulos

  • 00:07Introduction
  • 01:15Docker Desktop Installation
  • 04:25Quality Control
  • 05:10Mapping and Annotation Steps
  • 05:51Differentially Expressed Genes Process
  • 06:10Transfer Data to the Personal Computer
  • 12:02Representative Results
  • 14:22Conclusions

Summary

Tadução automática

Tadução automática

Den protokol, der præsenteres her, beskriver en komplet pipeline til at analysere RNA-sekventering af transskriptionomdata fra rå læser til funktionel analyse, herunder kvalitetskontrol og forbehandlingstrin til avancerede statistiske analytiske tilgange.

Tags

– High-throughput Transcriptome Analysis- Host-pathogen Interactions- Quality Control- Sequencing And Annotations- Statistical And Co-expression Analysis- Molecular Degree Of Perturbation Analysis- Functional Analysis- Docker Container- Chikungunya Virus- RNA Sequencing- Reference Genome- Gene Annotation

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