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用于研究宿主-病原体相互作用的高通量转录组分析
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
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用于研究宿主-病原体相互作用的高通量转录组分析

DOI:
10.3791/62324-v

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14:58 min

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March 05, 2022

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, , , , ,
1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School,University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences,University of São Paulo, 4Hospital Israelita Albert Einstein

Capítulos

  • 00:07Introduction
  • 01:15Docker Desktop Installation
  • 04:25Quality Control
  • 05:10Mapping and Annotation Steps
  • 05:51Differentially Expressed Genes Process
  • 06:10Transfer Data to the Personal Computer
  • 12:02Representative Results
  • 14:22Conclusions

Summary

Tadução automática

Tadução automática

这里介绍的方案描述了一个完整的管道，用于分析从原始读数到功能分析的RNA测序转录组数据，包括质量控制和预处理步骤到高级统计分析方法。

Tags

– High-throughput Transcriptome Analysis- Host-pathogen Interactions- Quality Control- Sequencing And Annotations- Statistical And Co-expression Analysis- Molecular Degree Of Perturbation Analysis- Functional Analysis- Docker Container- Chikungunya Virus- RNA Sequencing- Reference Genome- Gene Annotation

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