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In vitro kvantitativ billeddannelse assay for phagocytose af døde neuroblastom celler ved iPSC-makrofager
JoVE Journal
Neurociência
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JoVE Journal Neurociência
In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages

In vitro kvantitativ billeddannelse assay for phagocytose af døde neuroblastom celler ved iPSC-makrofager

DOI:
10.3791/62217-v

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09:48 min

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February 14, 2021

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, , , ,
1James Martin Stem Cell Facility, Sir William Dunn School of Pathology,University of Oxford, 2Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building,University of Oxford, 3UK Dementia Research Institute,Cardiff University

Capítulos

  • 00:04Introduction
  • 00:41Preparation of Dead SH-SY5Ys
  • 02:03Labeling of Dead SH-SY5Ys with pH-Sensitive Red Fluorescent Dye
  • 03:18Staining of iPSC-Macrophages
  • 04:50Fixed-Cell High-Content Imaging
  • 07:28Results: Imaging, Optimization and Validation of Phagocytosis Assay
  • 09:16Conclusion

Summary

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Tadução automática

Neurodegenerative sygdomme er forbundet med dysregulerede mikrogliafunktioner. Denne artikel skitserer en in vitro assay af fagocytose af neuroblastom celler ved iPSC-makrofager. Kvantitative udlæsninger af mikroskopi er beskrevet for både live-celle time-lapse imaging og fast celle højindhold billeddannelse.

Tags

Keywords: In VitroQuantitative Imaging AssayPhagocytosisDead Neuroblastoma CellsIPSC-macrophagesNeurodegenerative DiseaseSH-SY5Y CellsCell DissociationFixationPH-sensitive DyeFluorescent DyeMacrophage MediaHoechst 33342

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