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使用实时成像可视化氧化损伤对果蝇卵室的影响

JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento

Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging

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使用实时成像可视化氧化损伤对果蝇卵室的影响

DOI:

10.3791/62157-v

•

09:20 min

•

April 10, 2021

•

Kelsey M. Sheard, Rachel T. Cox

¹Department of Biochemistry and Molecular Biology,Uniformed Services University

Capítulos

00:00Introduction
01:14Preparing Single Ovarioles for Live Imaging
05:34H₂O₂ Treatment and Live Imaging
06:37Results: Representative Changes in Mitochondrial and Clu Bliss Particle Subcellular Localization After H₂O₂ Addition
08:25Conclusion

Summary

Tadução automática

该协议的目的是使用活成像来可视化氧化损伤对果蝇卵巢中亚细胞结构的定位和动力学的影响。

Tags

Drosophila Oxidative Damage Live Imaging Mitochondria Ribonucleoprotein Clu Schneider’s Drosophila Media Fetal Bovine Serum Pen-Strep Insulin Female Germ Cells Follicle Development Wet Yeast Paste

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