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一种基于板的测定法，用于测量急性脑切片中内源性单胺释放

JoVE Journal
Neurociência

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JoVE Journal Neurociência

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

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一种基于板的测定法，用于测量急性脑切片中内源性单胺释放

DOI:

10.3791/62127-v

•

07:56 min

•

August 11, 2021

•

Jose A. Pino*¹, Nora Awadallah*², Alessandra M. Norris, Gonzalo E. Torres

¹Department of Medicine, School of Medicine,Universidad de Atacama, ²Department of Molecular, Cellular, and Biomedical Sciences,City University of New York School of Medicine at City College, ³Department of Pharmacology and Therapeutics,University of Florida College of Medicine

Capítulos

00:04Introduction
00:44Acute Rat Brain Slice Preparation
02:01Ex Vivo Endogenous Monoamine Release Induction
04:42HPLC Analysis
05:29Results: Representative Endogenous Monoamine Release in Acute Brain Slices
07:21Conclusion

Summary

Tadução automática

该方法引入了一种使用急性脑切片检测内源性单胺释放的简单技术。该装置使用含有组织支架的48孔板进行单胺释放。释放的单胺通过HPLC结合电化学检测进行分析。此外，该技术还为药物发现提供了一种筛选方法。

Tags

Endogenous Monoamine Release Brain Slices Plate-based Assay Dorsal Striatum Hippocampus Efflux Buffer Pharmacological Conditions Vesicular Release Transporter-mediated Release

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