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Brug af immunofluorescence til at opdage PM2,5-induceret DNA-skade i Zebrafish Embryo Hearts
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts

Brug af immunofluorescence til at opdage PM2,5-induceret DNA-skade i Zebrafish Embryo Hearts

DOI:
10.3791/62021-v

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05:58 min

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February 15, 2021

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, , , , , , ,
1School of Public Health,Soochow University, 2Toxicology, Risk Assessment and Research Division,Texas Commission on Environmental Quality

Capítulos

  • 00:06Introduction
  • 00:34Morphological Observation of Zebrafish Embryos and Cardiac Dissection
  • 01:40Immunofluorescence Assay
  • 04:29Results: Cardiac Defects and DNA Damage of Zebrafish Embryos at 72 hpf
  • 05:36Conclusion

Summary

Tadução automática

Tadução automática

Denne protokol bruger en immunofluorescence assay til at opdage PM2,5-induceretDNA-skader i dissekeret hjerter zebrafisk embryoner.

Tags

Keywords: ImmunofluorescencePM2.5DNA DamageZebrafish EmbryoHeart8-hydroxydeoxyguanosineGamma-H2AXAntibodyFixationImmunostainingMicroscopy

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