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Kütle Spektrometresi ile Protein Yüksek Sıralı Yapısını incelemek için Diethylpyrocarbonate ile Kovalent Etiketleme
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JoVE Journal Química
Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry
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Kütle Spektrometresi ile Protein Yüksek Sıralı Yapısını incelemek için Diethylpyrocarbonate ile Kovalent Etiketleme

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10:36 min

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June 15, 2021

DOI:

10.3791/61983-v

DOI:
10.3791/61983-v

•

10:36 min
June 15, 2021

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2 Views
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1Department of Chemistry,University of Massachusetts Amherst, 2Molecular and Cellular Biology Program,University of Massachusetts Amherst, 3Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences,Chulalongkorn University

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Covalent LabelingDiethylpyrocarbonateDEPCMass SpectrometryProtein StructureStructural CharacterizationBuffer ExchangeImidazole

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Kirsch, Z. J., Arden, B. G., Vachet, R. W., Limpikirati, P. Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry. J. Vis. Exp. (172), e61983, doi:10.3791/61983 (2021).

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