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测量 果蝇 拉瓦的机械结节的改进的检测和工具
JoVE Journal
Comportamento
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JoVE Journal Comportamento
An Improved Assay and Tools for Measuring Mechanical Nociception in Drosophila Larvae
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测量 果蝇 拉瓦的机械结节的改进的检测和工具

DOI:
10.3791/61911-v

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07:17 min

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October 29, 2020

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1Department of Genetics,The University of Texas MD Anderson Cancer Center, 2Neuroscience Graduate Program, Graduate School of Biomedical Sciences,The University of Texas MD Anderson Cancer Center, 3Genetics and Epigenetics Graduate Program, Graduate School of Biomedical Sciences,The University of Texas MD Anderson Cancer Center

Capítulos

  • 00:04Introduction
  • 00:56Mechanical Probe Construction
  • 02:26Preparation of Larvae
  • 03:16Mechanical Nociception Assay
  • 04:33Results: Representative Mechanical Nociception and Hypersensitivity
  • 06:33Conclusion

Summary

Tadução automática

Tadução automática

该协议的目的是展示如何对 嗜血杆菌 幼虫的机械结节进行改进的检测。我们在这里使用测定来证明机械过敏（合金和高过敏症）存在于 嗜血杆菌 幼虫中。

Tags

Keywords: Mechanical NociceptionDrosophila LarvaeCustom ToolsNitinol FilamentsDose-response CurvesProbe CalibrationForce MeasurementPressure ConversionLarval SortingThird Instar Stage

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