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1Department General, Analytical and Physical Chemistry, Chair of General and Analytical Chemistry,Montanuniversität Leoben, 2Department of Forest and Soil Sciences, Institute of Soil Research, Rhizosphere Ecology and Biogeochemistry Group,University of Natural Resources and Life Sciences, Vienna, 3Department of Crop Sciences, Institute of Agronomy,University of Natural Resources and Life Sciences, Vienna
Capítulos
- 00:04Introduction
- 00:51Diffusive Gradients in Thin Films (DGT) Gel Fabrication and Rhizotron Preparation
- 03:06Plant Cultivation
- 03:47Solute Distribution Sampling
- 04:48Gel Retrieval and Chemical Analysis Preparation
- 05:38Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) Line Scan Analysis
- 07:39Data Processing and Calibration
- 09:03Image Generation
- 09:48Results: Representative Sub-mm 2D Solute Flux Distribution of Multiple Nutrients and Contaminants Across Different Soil-Root Interfaces
- 11:24Conclusion
Bu protokol, kütle spektrometresi görüntüleme ile birlikte ince filmlerdeki (DGT) difüzif gradyanlar kullanılarak birden fazla labile inorganik besin ve kirletici çözünür türünün mm altı 2D görselleştirilmesi için bir iş akışı sunar. Karasal bitkilerin rizosferinde solutların nicel haritalandırılması için çözünür örnekleme ve yüksek çözünürlüklü kimyasal analiz ayrıntılı olarak açıklanmıştır.
