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1Department General, Analytical and Physical Chemistry, Chair of General and Analytical Chemistry,Montanuniversität Leoben, 2Department of Forest and Soil Sciences, Institute of Soil Research, Rhizosphere Ecology and Biogeochemistry Group,University of Natural Resources and Life Sciences, Vienna, 3Department of Crop Sciences, Institute of Agronomy,University of Natural Resources and Life Sciences, Vienna
Capítulos
- 00:04Introduction
- 00:51Diffusive Gradients in Thin Films (DGT) Gel Fabrication and Rhizotron Preparation
- 03:06Plant Cultivation
- 03:47Solute Distribution Sampling
- 04:48Gel Retrieval and Chemical Analysis Preparation
- 05:38Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) Line Scan Analysis
- 07:39Data Processing and Calibration
- 09:03Image Generation
- 09:48Results: Representative Sub-mm 2D Solute Flux Distribution of Multiple Nutrients and Contaminants Across Different Soil-Root Interfaces
- 11:24Conclusion
Questo protocollo presenta un flusso di lavoro per la visualizzazione 2D sub-mm di più specie di nutrienti inorganici labili e soluti contaminanti utilizzando gradienti diffusivi in pellicole sottili (DGT) combinati con l'imaging della spettrometria di massa. Il campionamento del soluto e l'analisi chimica ad alta risoluzione sono descritti in dettaglio per la mappatura quantitativa dei soluti nella rizosfera delle piante terrestri.
