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1Department General, Analytical and Physical Chemistry, Chair of General and Analytical Chemistry,Montanuniversität Leoben, 2Department of Forest and Soil Sciences, Institute of Soil Research, Rhizosphere Ecology and Biogeochemistry Group,University of Natural Resources and Life Sciences, Vienna, 3Department of Crop Sciences, Institute of Agronomy,University of Natural Resources and Life Sciences, Vienna
Capítulos
- 00:04Introduction
- 00:51Diffusive Gradients in Thin Films (DGT) Gel Fabrication and Rhizotron Preparation
- 03:06Plant Cultivation
- 03:47Solute Distribution Sampling
- 04:48Gel Retrieval and Chemical Analysis Preparation
- 05:38Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) Line Scan Analysis
- 07:39Data Processing and Calibration
- 09:03Image Generation
- 09:48Results: Representative Sub-mm 2D Solute Flux Distribution of Multiple Nutrients and Contaminants Across Different Soil-Root Interfaces
- 11:24Conclusion
Dit protocol presenteert een workflow voor sub-mm 2D visualisatie van meerdere labiele anorganische nutriënten- en verontreinigingsolute soorten met behulp van diffusieve gradiënten in dunne films (DGT) in combinatie met massaspectrometrie beeldvorming. Solutebemonstering en chemische analyse in hoge resolutie worden in detail beschreven voor het kwantitatief in kaart brengen van soluten in de rhizosfeer van terrestrische planten.
