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通过微透析对芯片上的蛋白质进行结晶，用于 原位 X射线衍射研究
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Química
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JoVE Journal Química
Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
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通过微透析对芯片上的蛋白质进行结晶，用于 原位 X射线衍射研究

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12:38 min

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April 11, 2021

DOI:

10.3791/61660-v

DOI:
10.3791/61660-v

•

12:38 min
April 11, 2021

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1 Views
, , , , ,
1Université Grenoble Alpes, CEA, CNRS, IBS, 2CNRS, Solvay LOF, UMR 5258,Université Bordeaux, 3ELVESYS SAS

Tags

Protein CrystallizationMicrofluidic ChipsDialysisIn Situ X-ray DiffractionPDMSSU8 MasterRegenerated Cellulose Dialysis MembraneProtein Sample VolumeCrystallization ConditionsNucleationCrystal GrowthPhase Diagrams

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Jaho, S., Junius, N., Borel, F., Sallaz-Damaz, Y., Salmon, J., Budayova-Spano, M. Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies. J. Vis. Exp. (170), e61660, doi:10.3791/61660 (2021).

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