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Habilitación de la compensación en tiempo real en oxidaciones fotoquímicas rápidas de proteínas para la determinación de los cambios en la topografía proteica
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Bioquímica
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JoVE Journal Bioquímica
Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes
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Habilitación de la compensación en tiempo real en oxidaciones fotoquímicas rápidas de proteínas para la determinación de los cambios en la topografía proteica

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07:38 min

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September 01, 2020

DOI:

10.3791/61580-v

DOI:
10.3791/61580-v

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07:38 min
September 01, 2020

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1 Views
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1Department of Biomolecular Sciences,University of Mississippi, 2Department of Chemistry and Biochemistry,University of Mississippi, 3GenNext Technologies, Inc.

Tags

Real-time CompensationFast Photochemical OxidationsProtein Topography ChangesProtein ConfirmationProtein InteractionsBuffer CompositionSample PurityProtein ConcentrationDosimetry MethodDrug DevelopmentProtein StructureFPOP Optical BenchLaserInline DosimeterExcimer LaserMotorized StageHydrogen PeroxideFPOP Solution

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Misra, S. K., Sharp, J. S. Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes. J. Vis. Exp. (163), e61580, doi:10.3791/61580 (2020).

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